Single-cell transcriptomic analysis of Alzheimer's disease

Abstract summary

First large-scale single-nucleus RNA-seq study of the AD brain, profiling over 80,000 nuclei from 48 individuals. Identified disease-associated transcriptional changes across neurons, oligodendrocytes, astrocytes, and microglia. Microglial changes preceded and correlated with pathology progression. TREM2 and SPP1 were among the most differentially expressed microglial genes in AD.

Evidence labels

Therapeutic relevance

Identified cell-type-specific transcriptional changes in the AD brain, establishing microglial gene expression signatures as disease biomarkers.